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CRK12 and CYC9 interact in a yeast two hybrid assay. A: β-galactosidase assay for transcription of LacZLister 427 cells. Trypanosomes have been transfected with each plasmid independently, and then subjected into a 2nd transfection with one of the other plasmids, with a unique resistance gene, with all pairwise combos performed.In contrast, any ti